Journal: STAR Protocols
Article Title: Transmission electron microscopic analysis of myelination in the murine central nervous system
doi: 10.1016/j.xpro.2022.101304
Figure Lengend Snippet: Illustration of tissue sectioning and embedding (A) Brain sectioning. (A1) Brain in a brain slicer matrix. Arrow shows the true lambda . (A2) Brain was cut into 1 mm slices with a brain slicer matrix and razor blades. (A3) Three coronal sections of the mouse brain. The second section was used for corpus callosum analysis. The line shows the edge of corpus callosum. (A4) Trimming of the brain to obtain the region of interest, corpus callosum. (A5) Enlarged picture of Figure A4. The line shows the edge of corpus callosum. (A6) Embedding mark cut at the outer edge of the corpus callosum. (B) Separated right and left optic nerves using a razor blade. (C) Spinal cord sectioning. (C1) Spinal cord was cut into 1 mm slices at the region of lumbar enlargement with a spinal cord slicer matrix and razor blades. (C2) Four coronal sections of spinal cord. (D) Immersion fixation of tissues sections in 3% glutaraldehyde at 4°C in a refrigerator overnight. (E–G) Resin embedding of the corpus callosum, optic nerve and spinal cord. Small, printed labels are included in the blocks to identify the sample. Scale bars: (A3, A4, B and C2) 0.5 cm; (A5 and A6) 0.25 cm; (E–G) 1 cm.
Article Snippet: Acrylic mouse brain matrix, coronal, 1 mm , Braintree Scientific, Inc , BS-A-5000C.
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